You would get a lot of differences, but the errors would cancel each other out with enough depth of coverage.
replyThis technology's baseline accuracy is around 95% per base, so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other.
> so 10x reads of every segment in the sample would give >99% accuracy for each base after aligning the reads with each other
replyThis assumes random errors, which IIRC isn't the case for Oxford Nanopore.
Oxford Nanopore unfortunately has a high error rate (3-5%) compared to other sequencing technologies. And the errors are non-random
replyThis is simply not true, advances in basecalling and using Duplex give you >Q25
replyFor most DNA it now sits at ~Q20 (1%) which is a big improvement - there was also some hacky stuff like HERRO and duplex sequencing which improved it substantially. For routine genome sequencing it's definitely suitable, and the long-read advantage helps resolve some of those trickier regions
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