How many simultaneous mosquitos in Singapore?

lets say on the order of 1 billion, and let's assume the effectivity doesn't change when considering the 30% unaffected population

it would need to be applied to the surviving 30% repeatedly:

0.3 ^ N = 1 / 1B

so N = log(1B)/log(10/3) = ~17.2 times.

I predict nature protects itself with diversity: some of the male and female wild type will have natural aversion from, attraction to or neutral stance the modified Wolbachia carriers.

I predict that those mosquito family lines that happen to be more Wolbachia-carrier averse become enriched over time.

There is an energetic cost to maintaining a parasitic lifeform, and that energetic term will always favor immunity against the parasite.